The Mii Brawler puppet fighter represents Cap'n Cuttlefish, an elderly Inkling who serves as the player's guide in the Splatoon series' story modes.
The Mii Brawler puppet fighter's Vince's Hat references Cap'n Cuttlefish's white beard and old captain's hat, while the Skull Kid's Outfit references Cap'n Cuttlefish's ragged clothes.
The stage represents Octo Valley, the main hub area of Splatoon's similarly-named story mode where the player first encounters Cap'n Cuttlefish in a valley.
The music track is based on the final boss theme song sung by Cap'n Cuttlefish's granddaughters, the Squid Sisters, who played for him to break free of DJ Octavio's restraints during the latter's boss battle.
The Mii Brawler starts the battle with a Ray Gun, referencing both the different Splattershot weapons and the shooting gameplay mechanic in the Splatoon series.
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