The yellow Isabelle and the Flowery rule represent Crazee Dayzee, a smiling flower who features yellow petals.
The stage is based on Yoshi's Island, the main setting of Super Mario World 2: Yoshi's Island, which first features Crazee Dayzee as an enemy.
The Meloetta Poké Ball Pokémon's Echoed Voice ability references the musical notes that accompany Crazee Dayzee.
Other trivia:
This spirit's Weight ↓ ability references how the Crazee Dayzee is often seen skipping along in its game appearances.
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These flowery fellows are almost identical to Shy Guys in every way but appearance, only being able to walk in Yoshi’s direction. The one difference is that they can be swallowed and spit out, no problem. You’ll have to throw them into another enemy if you want one gone.