The blue Samus represents her appearance in Metroid Dread, who wears a Power Suit with a blue color scheme.
The stage represents the planet ZDR, the main setting of Metroid Dread, which features research facilities and laboratories similar to the Frigate Orpheon.
The Metroid Assist Trophy enemy references how Samus possesses the Metroid's DNA after the events of Metroid Fusion, which serves as the prequel to Metroid Dread.
Other trivia:
This spirit's Shooting Items Power ↑ ability increases the power of the player's shooting items, referencing how Samus receives upgrades for her Power Suit, including her Beam and Missile weapons.
