The purple Dark Samus represents the Zebesian, a Space Pirate depicted as a purple humanoid enemy who appears multiple times on-screen.
The stage and music track are based on Brinstar, an area in Super Metroid, which features the first appearance of the Zebesian as an enemy.
Dark Samus's usage of Charge Shot and the battle condition reference how the Zebesian attacks by firing lasers from their claws.
Other trivia:
This spirit's Fist Attack ↑ ability increases the power of punches, referencing how the Zebesian attack by using their claws.
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The basic Space Pirate, the rebuilt base on Zebes was crawling with these guys in Super Metroid. There are all kinds of different variations, but all of them can cling to walls. In Metroid: Other M, the Federation created cyberized versions of them, equipped with robotic weaponry. When infected with the X Parasite, Zebesians gain the ability to swim.
